thr77 login Options
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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has become observed in several tumors, which leads to chromosome instability, and therefore might lead to tumorigenesis. This protein was observed to associate with, and become linked to, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and performs a essential function in promoting mobile-cycle progression during the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are actually described. Two extra splice variants were being claimed but in-depth nucleotide sequence information and facts is not really but offered.
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Blocking peptides are peptides that bind particularly to the goal antibody and block antibody binding. These peptide typically has the epitope recognized through the antibody.
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This information outlines more achievable optimization of mobile and immunoassay parameters to guarantee the absolute best outcomes are...
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This guide outlines additional feasible optimization of cellular and immunoassay parameters to make certain the absolute best final results are...
Antibodies certain to the blocking peptide not bind for the epitope about the goal protein. By comparing the staining in the blocked antibody versus the antibody alone, you can see which staining is particular.
Goal Info Cyclin E belongs towards the remarkably conserved cyclin spouse and children, whose customers are characterized by a remarkable periodicity in protein abundance from the mobile cycle. Cyclins function as regulators of CDK kinases. Distinctive cyclins show unique expression and degradation designs which contribute to the temporal coordination of each and every mitotic function. Cyclin E types a posh with and features like a regulatory subunit of CDK2, whose activity is necessary for mobile cycle G1/S transition. This protein accumulates with the G1-S section boundary and is particularly degraded as cells development by S stage.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both of those antibodies...
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